Journal: Scientific Reports

Article Title: Downregulation of miR-335-5P in Amyotrophic Lateral Sclerosis Can Contribute to Neuronal Mitochondrial Dysfunction and Apoptosis

doi: 10.1038/s41598-020-61246-1

Figure Lengend Snippet: Deregulated miRNA expression in the discovery phase. Normalized relative expression levels of serum miRNAs in ALS patients compared to healthy controls. Data are presented as mean ± SD. *p ≤ 0.05 (Mann-Whitney U test). Graphics of miRNA expression were created using GraphPad Prism, version 5.03 ( https://www.graphpad.com/scientific-software/prism/ ).

Article Snippet: The graphic representations were created using GraphPad Prism, version 5.03, ( https://www.graphpad.com/scientific-software/prism/ ).

Techniques: Expressing, MANN-WHITNEY, Software

Journal: Scientific Reports

Article Title: Downregulation of miR-335-5P in Amyotrophic Lateral Sclerosis Can Contribute to Neuronal Mitochondrial Dysfunction and Apoptosis

doi: 10.1038/s41598-020-61246-1

Figure Lengend Snippet: MiRNA expression in validation phase. Only miR-335-5p expression showed statistically significant differences between ALS patients and healthy controls. Normalized relative expression levels of serum miRNAs in ALS patients compared to healthy controls. Data are presented as mean ± SD. *p ≤ 0.05 (Mann-Whitney U test). Graphics of miRNA expression were created using GraphPad Prism, version 5.03 ( https://www.graphpad.com/scientific-software/prism/ ).

Article Snippet: The graphic representations were created using GraphPad Prism, version 5.03, ( https://www.graphpad.com/scientific-software/prism/ ).

Techniques: Expressing, MANN-WHITNEY, Software

Journal: Scientific Reports

Article Title: Downregulation of miR-335-5P in Amyotrophic Lateral Sclerosis Can Contribute to Neuronal Mitochondrial Dysfunction and Apoptosis

doi: 10.1038/s41598-020-61246-1

Figure Lengend Snippet: Co-transfection of miR-335 inhibitor or NC together with Keima Red. ( A ) Confocal images showed an increased pool of mitochondria 48 and 72 h after transfection with the miR-335 inhibitor.0 ( B ) 561/458 nm excitation ratio showed an increase number of red mitochondria (excited at 561), indicating there localization in an acidic environment. The graphic representation was created using GraphPad Prism, version 5.03, ( https://www.graphpad.com/scientific-software/prism/ ).

Article Snippet: The graphic representations were created using GraphPad Prism, version 5.03, ( https://www.graphpad.com/scientific-software/prism/ ).

Techniques: Cotransfection, Transfection, Software

Journal: Scientific Reports

Article Title: Downregulation of miR-335-5P in Amyotrophic Lateral Sclerosis Can Contribute to Neuronal Mitochondrial Dysfunction and Apoptosis

doi: 10.1038/s41598-020-61246-1

Figure Lengend Snippet: Mitochondria co-localize with p62 after miR-335 inhibitor transfection. ( A ) Co-localization of mitochondria (red) with p62 autophagic marker (green), 72 h after transfection with miR-335 inhibitor. ( B ) Representative WB of p62 in cells after 24, 48 or 72 hours after transfection. Bands corresponding to p62 and β-actin were cropped from the same gel. ( C ) WB quantification showed a decrease in p62 expression at 48 h, and an increase at 72 h after transfection, indicating potential impairment of autophagy in transfected cells. The graphic representation was created using GraphPad Prism, version 5.03, ( https://www.graphpad.com/scientific-software/prism/ ).

Article Snippet: The graphic representations were created using GraphPad Prism, version 5.03, ( https://www.graphpad.com/scientific-software/prism/ ).

Techniques: Transfection, Marker, Expressing, Software

Journal: Scientific Reports

Article Title: Downregulation of miR-335-5P in Amyotrophic Lateral Sclerosis Can Contribute to Neuronal Mitochondrial Dysfunction and Apoptosis

doi: 10.1038/s41598-020-61246-1

Figure Lengend Snippet: Increment of ROS and SOD activity after miR-335 inhibitor transfection. ( A ) ROS detection in SH-SY5Y transfected cells 24 h after transfection correlated with an increment of SOD activity as a measure to regulate ROS levels ( B ). The graphic representation was created using GraphPad Prism, version 5.03, ( https://www.graphpad.com/scientific-software/prism/ ).

Article Snippet: The graphic representations were created using GraphPad Prism, version 5.03, ( https://www.graphpad.com/scientific-software/prism/ ).

Techniques: Activity Assay, Transfection, Software

Journal: Scientific Reports

Article Title: Downregulation of miR-335-5P in Amyotrophic Lateral Sclerosis Can Contribute to Neuronal Mitochondrial Dysfunction and Apoptosis

doi: 10.1038/s41598-020-61246-1

Figure Lengend Snippet: Abnormal activity of apoptosis effector caspases after miR-335 inhibitor transfection. ( A ) Representative WB of caspase-3 and 7 expressions after miR-335 transfection. Bands corresponding to caspase-7, caspase-3 and β-actin were cropped from the same gel. We can quantify the bands corresponding to caspase-7 and caspase-3 (B), and although no significant differences were observed, when we measured the activity of these caspases, we observed a significant increase in caspase activity (**p < 0.01) ( C ). The graphic representations were created using GraphPad Prism, version 5.03, ( https://www.graphpad.com/scientific-software/prism/ ).

Article Snippet: The graphic representations were created using GraphPad Prism, version 5.03, ( https://www.graphpad.com/scientific-software/prism/ ).

Techniques: Activity Assay, Transfection, Software

Journal: The Journal of Biological Chemistry

Article Title: Conformationally Trapping the Actin-binding Cleft of Myosin with a Bifunctional Spin Label *

doi: 10.1074/jbc.M112.428565

Figure Lengend Snippet: Bifunctional spin labeling. A, Dicty myosin II catalytic domain crosslinked by a BSL, shown in red (space-filling), between engineered cysteine residues 416 and 583 in the U50 (green) and L50 (blue) domains of the actin-binding cleft. BSL was modeled on crystal structure 1FMV using Discovery Studio 2.5.5 and visualized using PyMOL. B, conventional (V1) EPR spectrum of the labeled protein in solution.

Article Snippet: Molecular Modeling Spin labels were modeled on crystal structure 1FMV using Discovery Studio 2.5.5 (Accelrys Software Inc., San Diego) and visualized using the PyMOL Molecular Graphics System, Version 1.5.0.3 (Schrodinger, LLC).

Techniques: Labeling, Binding Assay